Review

goat anti pdgfr β (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems goat anti pdgfr β
Goat Anti Pdgfr β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+pdgfr+%CE%B2/Mouse+PDGF+R+beta+Antibody/pmc13124745-67-41-45
Average 96 stars, based on 237 article reviews

goat anti pdgfr β - by Bioz Stars, 2026-10

96/100 stars

Images

other:

Article Title: Depletion of BIS in astrocytes aggravates reactive gliosis following photothrombotic brain injury.
Article Snippet: The following primary antibodies were used: rabbit anti-BIS [1] (1:1,000), rabbit anti-BAG3 (1:200, Proteintech, 10599-1-AP), chicken anti-GFAP (1:700, Merck Millipore, AB5541), guinea pig anti-Iba1 (1:400, Synaptic Systems, 234 308), rabbit anti-Lamp1 (1:400, Abcam, ab24170), mouse antinestin (1:500, AbD Serotec, 6625-1010), goat anti-PDGFR-β (1:100, R&D systems, AF1042), and mouse anti-p62 (1:100, Abcam, ab56416).

Article Title: Endothelial delivery of simvastatin by LRP1-targeted nanoparticles ameliorates pathogenesis of alzheimer’s disease in a mouse model
Article Snippet: Rabbit anti-LRP1 (ab92544, Abcam), Rat anti-CD31 (550274, BD), Mouse anti-NeuN (94403, CST), Rat anti-NeuN (ab279297, Abcam), Rabbit-anti-GFAP (3670T, CST), Goat-anti-IBA-1 (AB5076, Abcam), Goat anti-PDGFR β (AF385, R&D systems), Mouse anti-A β -6E10 (803015, biolegend), Rabbit anti-synaptophysin (SYN) (ab32127, Abcam), Rabbit anti-ZO1 (ab276131, Abcam), Rabbit anti-Occludin (ab216327, Abcam), Rabbit anti-Claudin 5 (AF5216, AffinitY), Rabbit anti-RAGE (ab216329, Abcam), Mouse anti-APP (MAB348, Merke), Mouse anti-BACE1 (NBP2-37261, Novus), Mouse anti-GAPDH (FD0063-100, FD), β -actin(66009-1, Proteintech), Alexa Fluor 555 Donkey anti-Rabbit (A32794, Invitrogen), Alexa Fluor 488 Donkey anti Rabbit (A21206, Invitrogen), Alexa Fluor 555 Donkey anti Mouse (A32773, Invitrogen), Alexa Fluor 488 Donkey anti Mouse (A32766, Invitrogen), Alexa Fluor 488 Donkey anti Goat (A11055, Invitrogen), Alexa Fluor 488 Donkey anti-Rat (A21208, Invitrogen), and HRP AffiniPure Goat anti-Mouse IgG (H + L) (FD0142, FD), HRP AffiniPure Goat anti-Rabbit IgG (H + L) (FD0128, FD) were used.

Article Title: Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions
Article Snippet: Novus Biologicals, Littleton, CO); rabbit anti-RFP (1:1000, Cat#600-401-379, Rockland, Limerick, PA); goat anti-Albumin (1:300, Cat#NB600-41532, Novus Biologicals, Littleton, CO); rat anti-PECAM-1 (1:200, Cat#550274, clone-MEC 13.3 BD Biosciences, San Jose, CA); rat anti-Galectin-3 (1:200, Cat#14-5301-82, Clone-M3/38, Invitrogen-Thermofisher Scientific, Grand Island, NY); rat anti-CD68 (1:1000, Cat# MCA1957, CLone-FA-11, AbDserotec-BioRad, Hercules, CA); rat anti-CD45 (1:100, Cat#553076, Clone-30-F11, BD Biosciences, San Jose, CA); rabbit anti-Iba-1 (1:800, Cat#019-19741, Wako, Osaka, Japan); guinea pig anti-Iba-1 (1:800, Cat#234-004, Synaptic systems, Goettingen, Germany); Rabbit anti-P2Y12R (1:500, Cat#AS-55043A, Anaspec, Fremont, CA); rabbit anti-mouse IgG (1:1000, Cat#97042, Abcam, Cambridge, MA); rat anti-Ly6B2 (1:200, Cat#MCA771GT, Clone-7/4, BioRad, Hercules, CA); Goat anti-PDGFR-β (1:200, Cat#AF1042, R&D systems, Minneapolis, MN); and rabbit anti-Olig2 (1:200, Cat#AB9610, Millipore, Burlington, MA).

Article Title: Depletion of BIS in astrocytes aggravates reactive gliosis following photothrombotic brain injury
Article Snippet: The following primary antibodies were used: rabbit anti-BIS [ ] (1:1,000), rabbit anti-BAG3 (1:200, Proteintech, 10599-1-AP), chicken anti-GFAP (1:700, Merck Millipore, AB5541), guinea pig anti-Iba1 (1:400, Synaptic Systems, 234 308), rabbit anti-Lamp1 (1:400, Abcam, ab24170), mouse anti-nestin (1:500, AbD Serotec, 6625-1010), goat anti-PDGFR- β (1:100, R&D systems, AF1042), and mouse anti-p62 (1:100, Abcam, ab56416).

Article Title: Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice
Article Snippet: The primary antibodies used in this study included the following: goat anti-mCherry (Biorbyt, Cat#: Orb11618, Lot#: J2446; 1:500), rat anti-CD31 (BD Biosciences, Cat#: 550274, Lot#: 9259767; 1:10), rabbit anti-IBA1 (Wako, Cat#: 019-19741 Lot#: WDK2121; 1:500), goat anti-IBA1 (Abcam, Cat#: ab5076, Lot#: GR3187278-2; 1:500), chicken anti-GFP (Abcam, Cat#: ab13970, Lot#: GR236651-12; 1:1,000), rabbit anti-S100β (Abcam, Cat#: ab52642, Lot#: GR252937-5; 1:300), rabbit anti-GFAP (Abcam, Cat#: ab7260, Lot#: GR297722-2; 1:300), rabbit anti-α-SMA (Abcam, Cat#: ab124964, Lot#: GR181740-66; 1:300), mouse anti-GFAP (Sigma‒Aldrich, Cat#: G3893, Lot#: 105M4784 V; 1:200), goat anti-PDGFR-β (R&D systems, Cat#: AF385, Lot#: BIWO619121; 1:200), rabbit anti-LC3 (Cell Signaling Technologies, Cat#: 4108, Lot#: 3; 1:100), rat anti-LAMP1 (1D4B) (Santa Cruz, Cat#: SC-19992, Lot#: C2715; 1:50), rabbit anti-PDGFR-α (Cell Signaling Technologies, Cat#: 3174 s, Lot#: 7; 1:500), mouse anti-CC1 (Millipore, Cat#: 14-0661-82, Lot#: 3129980; 1:200), rat anti-mouse C4 (Abcam, Cat#: ab11863, Lot#: GR3315169-2; 1:100), goat anti-GFP (Abcam, Cat#: AB6673, Lot#: GR3373716-2; 1:1,000), chicken anti-mCherry (Abcam, Cat#: ab205402, Lot#: GR3271744-8; 1:500), chicken anti-NESTIN (Abcam, Cat#: ab134017, Lot#: GR3291127-1; 1:200), rabbit anti-RFP (Abcam, Cat#: ab62341, Lot#: GR3319727-1; 1:1,000), rabbit anti-NeuN (Abcam, Cat#: ab177487, Lot#: GR3275122-6; 1:500), rabbit anti-MBP (Abcam, Cat#: ab40390, Lot#: GR297609-1; 1:200), rabbit anti-LAMININ (Sigma, Cat#: L9393-100UL, Lot#: 087M4889 V; 1:250), rabbit anti-GFP (Invitrogen, Cat#: A-11122, Lot#: 2273763; 1:1000), goat anti-OLIG2 (R&D, Cat#: AF2418, Lot#: UPA0719061; 1:400) and rabbit anti-PDGFα (Cell Signaling, Cat#: 3164 S, Lot#: 02/2020-6; 1:500).

Incubation:

Article Title: Microglia control cerebral blood flow and neurovascular coupling via P2Y12R-mediated actions
Article Snippet: For high resolution confocal laser scanning microscopy (CLSM) and electron microscopic assessments, 50 μm thick vibratome sections were washed in PB and TBS, followed by blocking with 1% human serum albumin (HSA). .. Sections were then incubated in different mixtures of primary antibodies: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFAP (1:500, #173 006 Synaptic Systems), goat anti-PDGFR-β (1:500, #AF1042 R&D Systems), rat anti-CD206 (1:200, #MCA2235, AbD Serotec), rat anti-PECAM-1 (1:500, #102 501, BioLegend), mouse anti-αSMA (1:250, #ab7817, Abcam), guinea pig anti-Aquaporin-4 (1:500, #429 004, Synaptic Systems), mouse anti-TOM20 (1:500, #H00009804-M01, Abnova), guinea pig anti-Iba1 (1:500, #234 004, Synaptic Systems), mouse anti-Kv2.1 (1:500, #75-014, NeuroMab) and biotinylated tomato lectin (1:100, #B-1175, Vectorlabs). .. After washing in TBS, sections were incubated in the corresponding mixtures of secondary antibodies: donkey anti-chicken DyLight405 (1:500, #703-474-155, Jackson ImmunoResearch), donkey-anti chicken A488 (1:500, #703-546-155, Jackson ImmunoResearch), donkey anti-chicken A647 (1:500, #703-606-155, Jackson ImmunoResearch), donkey anti-rabbit A647 (1:500, #711-605-152, Jackson ImmunoResearch), donkey anti-rabbit A488 (1:500, #A21206, Invitrogen), donkey anti-rat A594 (1:500, #A21209, Invitrogen), donkey anti-rat A647 (1:500, #712-606-153, Jackson ImmunoResearch), donkey anti-mouse A594 (1:500, #A21203, Invitrogen), donkey anti-mouse A647 (1:500, #715-605-150, Jackson ImmunoResearch), donkey anti-guinea pig DyLight405 (1:500, #706-476-148, Jackson ImmunoResearch), donkey anti-guinea pig A594 (1:500, #706-586-148, Jackson ImmunoResearch), donkey anti-guinea pig A647 (1:500, #706-606-148, Jackson ImmunoResearch), streptavidin DyL405 (1:500, #016-470-084, Jackson ImmunoResearch), streptavidin A594 (1:500, #S11227, Invitrogen).

Article Title: Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Article Snippet: .. Sections were then incubated in different mixtures of primary antibodies: rabbit anti-P2Y12R (1:500; #55043AS; AnaSpec), chicken antiGFAP (1:500; #173 006; Synaptic Systems), goat anti-PDGFR-β (1:500; #AF1042; R&D Systems), rat anti-CD206 (1:200; #MCA2235; AbD Serotec), rat anti-PECAM-1 (1:500; #102 501; BioLegend), mouse anti-αSMA (1:250; #ab7817; Abcam), guinea pig anti-AQP4 (1:500; #429 004; Synaptic Systems), mouse anti-TOM20 (1:500; #H00009804-M01; Abnova), guinea pig anti-Iba1 (1:500; #234 004; Synaptic Systems), mouse anti-Kv2.1 (1:500; #75-014; NeuroMab), and biotinylated tomato lectin (1:100; #B-1175; Vectorlabs). .. After washing in TBS, sections were incubated in the corresponding mixtures of secondary antibodies (from Jackson ImmunoResearch): donkey anti-chicken DyLight405 (1:500; #703-474- 155), donkey anti-chicken A488 (1:500; #703-546-155), donkey anti-chicken A647 (1:500; #703-606-155), donkey anti-rabbit A647 (1:500; #711-605-152), donkey anti-rabbit A488 (1:500; #A21206; Invitrogen), donkey anti-rat A594 (1:500; #A21209; Invitrogen), donkey anti-rat A647 (1:500; #712-606-153), donkey anti-mouse A594 (1:500; #A21203; Invitrogen), donkey antimouse A647 (1:500; #715-605-150), donkey anti–guinea pig DyLight405 (1:500; #706-476-148), donkey anti–guinea pig A594 (1:500; #706-586-148), donkey anti–guinea pig A647 (1:500; #706-606-148), streptavidin DyL405 (1:500; #016-470-084), and streptavidin A594 (1:500; #S11227; Invitrogen).

Article Title: Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions
Article Snippet: .. Sections were then incubated in different mixtures of primary antibodies: rabbit anti-P2Y12R (1:500; #55043AS; AnaSpec), chicken anti-GFAP (1:500; #173 006; Synaptic Systems), goat anti-PDGFR-β (1:500; #AF1042; R&D Systems), rat anti-CD206 (1:200; #MCA2235; AbD Serotec), rat anti-PECAM-1 (1:500; #102 501; BioLegend), mouse anti-αSMA (1:250; #ab7817; Abcam), guinea pig anti-AQP4 (1:500; #429 004; Synaptic Systems), mouse anti-TOM20 (1:500; #H00009804-M01; Abnova), guinea pig anti-Iba1 (1:500; #234 004; Synaptic Systems), mouse anti-Kv2.1 (1:500; #75-014; NeuroMab), and biotinylated tomato lectin (1:100; #B-1175; Vectorlabs). .. After washing in TBS, sections were incubated in the corresponding mixtures of secondary antibodies (from Jackson ImmunoResearch): donkey anti-chicken DyLight405 (1:500; #703-474-155), donkey anti-chicken A488 (1:500; #703-546-155), donkey anti-chicken A647 (1:500; #703-606-155), donkey anti-rabbit A647 (1:500; #711-605-152), donkey anti-rabbit A488 (1:500; #A21206; Invitrogen), donkey anti-rat A594 (1:500; #A21209; Invitrogen), donkey anti-rat A647 (1:500; #712-606-153), donkey anti-mouse A594 (1:500; #A21203; Invitrogen), donkey anti-mouse A647 (1:500; #715-605-150), donkey anti–guinea pig DyLight405 (1:500; #706-476-148), donkey anti–guinea pig A594 (1:500; #706-586-148), donkey anti–guinea pig A647 (1:500; #706-606-148), streptavidin DyL405 (1:500; #016-470-084), and streptavidin A594 (1:500; #S11227; Invitrogen).

Image Search Results

Conditional knockout of Tgfβ1 in macrophage lineage cells reduces pSmad2 levels and promotes functional recovery in SCI mice. a – d ELISA analysis showing the concentration of TGF-β in the spinal cord and the serum after SCI in each mouse for the groups indicated (* P < 0.05, ** P < 0.01, n = 4). e Representative Western blots showing the activation of phosphorylated Smad (pSmad) signaling. f Representative images of immunofluorescent staining of pSmad2 + (red), platelet-derived growth factor receptor-β + (PDGFR-β + ) (green) pericytes , and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. g Representative immunofluorescence images of PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 2 weeks after SCI. Scale bars, 200 µm. h Quantitative analysis of the number of pSmad2 + cells (**** P < 0.000 1, n = 6). i Quantitative analysis of the number of PDGFR-β + cells (** P < 0.01, n = 6). j , k Illustrations of the hot plate test and the von Frey test. l Quantitative analysis of hindpaw withdrawal time responding to temperature (hot plate test, ** P < 0.01, n = 8). m , n Quantitative analysis of hindpaw withdrawal frequency responding to mechanical stimulation (von Frey test, 0.7 mN and 3.9 mN, * P < 0.05, ** P < 0.01, n = 8). o Quantitative analysis of Basso Mouse Scale (BMS) score between Tgfb1 flox / flox control mice after SCI, Tgfb1 LysM-cre −/− mice after SCI, and Tgfb1 flox/flox control mice without SCI (* P < 0.05, n = 8). PWF paw withdrawal frequency. LF left forepaw, RF right forepaw, RH right hindpaw. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Journal: Bone Research

Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

doi: 10.1038/s41413-026-00507-7

Figure Lengend Snippet: Conditional knockout of Tgfβ1 in macrophage lineage cells reduces pSmad2 levels and promotes functional recovery in SCI mice. a – d ELISA analysis showing the concentration of TGF-β in the spinal cord and the serum after SCI in each mouse for the groups indicated (* P < 0.05, ** P < 0.01, n = 4). e Representative Western blots showing the activation of phosphorylated Smad (pSmad) signaling. f Representative images of immunofluorescent staining of pSmad2 + (red), platelet-derived growth factor receptor-β + (PDGFR-β + ) (green) pericytes , and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. g Representative immunofluorescence images of PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 2 weeks after SCI. Scale bars, 200 µm. h Quantitative analysis of the number of pSmad2 + cells (**** P < 0.000 1, n = 6). i Quantitative analysis of the number of PDGFR-β + cells (** P < 0.01, n = 6). j , k Illustrations of the hot plate test and the von Frey test. l Quantitative analysis of hindpaw withdrawal time responding to temperature (hot plate test, ** P < 0.01, n = 8). m , n Quantitative analysis of hindpaw withdrawal frequency responding to mechanical stimulation (von Frey test, 0.7 mN and 3.9 mN, * P < 0.05, ** P < 0.01, n = 8). o Quantitative analysis of Basso Mouse Scale (BMS) score between Tgfb1 flox / flox control mice after SCI, Tgfb1 LysM-cre −/− mice after SCI, and Tgfb1 flox/flox control mice without SCI (* P < 0.05, n = 8). PWF paw withdrawal frequency. LF left forepaw, RF right forepaw, RH right hindpaw. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Article Snippet: The sections were incubated with primary antibodies to rabbit 5-HT (1:50, sc-65495, Santa Cruz Biotechnology, Dallas, TX, USA), mouse β-III-tubulin (1:100, MA1-118, Invitrogen, Carlsbad, CA, USA), rabbit PGP9.5 (1:250, ab108986, Abcam, Cambridge, UK), mouse PGP9.5 (1:50, ab8189, Abcam), rabbit Fibronectin (1:100, ab2413, Abcam), mouse Fibronectin (1:100, ab6328, Abcam), rabbit Collagen III (1:100, ab7778, Abcam), chicken GFAP (1:500, ab4674, Abcam), mouse Collagen1α1 (1:50, sc-293182, Santa Cruz Biotechnology), rabbit Phospho SAMD2 (1:100, 44-244 G, Invitrogen), rabbit PDGFR-β (1:100, ab32570, Abcam), goat PDGFR-β (1:100, AF1042, R&D Systems), rabbit FSP1 (1:300, 07-2274, MilliporeSigma, Burlington, MA, USA), and chicken green fluorescent protein (1:250, ab13970, Abcam) overnight at 4 °C.

Techniques: Knock-Out, Functional Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Western Blot, Activation Assay, Staining, Derivative Assay, Immunofluorescence, Hot Plate Test, Control

Conditional knockout of TGF-β type 2 receptor ( Tgfbr2 ) in pericytes reduces fibrotic scar formation in SCI mice. a Schematic diagram of Tgfbr2 knockout in Glast-Cre + pericytes. b Representative images of immunofluorescent analysis of PGP9.5 + (red) nerve fibers, fibronectin + (green) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. c , d Quantitative analysis of the intensity value of PGP9.5 and fibronectin (* P < 0.05, n = 6). e Representative images of immunofluorescent analysis of nerve axon–specific β-III-tubulin + (green), collagen III + (red) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. Right images are high resolution versions of the boxed regions in the left images. Scale bar, 50 µm. f Quantitative analysis of the intensity value of collagen III (* P < 0.05, n = 6). g Representative images of immunofluorescent analysis of neurotransmitter marker 5-HT + (red), nerve axon–specific β-III-tubulin + (green), and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in Tgfbr2 flox/flox sham group mice, Tgfbr2 flox/flox control mice, and Tgfbr2 Glast-creER −/− mice at 4 weeks after SCI. Scale bar, 200 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. h , i Quantitative analysis of the intensity value of 5-HT and β-III-tubulin (* P < 0.05, n = 6). j Representative images of immunofluorescent analysis of pSmad2 + (red), PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. k , l Quantitative analysis of the intensity mean value of PDGFR-β and the number of pSmad2 + cells (*** P < 0.001, **** P < 0.000 1, n = 6). m Representative Western blots showing the activation of pSmad signaling. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Journal: Bone Research

Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

doi: 10.1038/s41413-026-00507-7

Figure Lengend Snippet: Conditional knockout of TGF-β type 2 receptor ( Tgfbr2 ) in pericytes reduces fibrotic scar formation in SCI mice. a Schematic diagram of Tgfbr2 knockout in Glast-Cre + pericytes. b Representative images of immunofluorescent analysis of PGP9.5 + (red) nerve fibers, fibronectin + (green) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. c , d Quantitative analysis of the intensity value of PGP9.5 and fibronectin (* P < 0.05, n = 6). e Representative images of immunofluorescent analysis of nerve axon–specific β-III-tubulin + (green), collagen III + (red) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. Right images are high resolution versions of the boxed regions in the left images. Scale bar, 50 µm. f Quantitative analysis of the intensity value of collagen III (* P < 0.05, n = 6). g Representative images of immunofluorescent analysis of neurotransmitter marker 5-HT + (red), nerve axon–specific β-III-tubulin + (green), and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in Tgfbr2 flox/flox sham group mice, Tgfbr2 flox/flox control mice, and Tgfbr2 Glast-creER −/− mice at 4 weeks after SCI. Scale bar, 200 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. h , i Quantitative analysis of the intensity value of 5-HT and β-III-tubulin (* P < 0.05, n = 6). j Representative images of immunofluorescent analysis of pSmad2 + (red), PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. k , l Quantitative analysis of the intensity mean value of PDGFR-β and the number of pSmad2 + cells (*** P < 0.001, **** P < 0.000 1, n = 6). m Representative Western blots showing the activation of pSmad signaling. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Techniques: Knock-Out, Staining, Marker, Control, Western Blot, Activation Assay

LepR + MSCs are the primary cells for fibrotic scar formation. a , b Diagram illustrating the genetic strategy to trace type A pericytes and the timeline of tamoxifen injection. c Representative images of immunofluorescent analysis of tdT + (red) type A pericytes, PDGFR-β + (green) pericytes, and DAPI (blue) staining of nuclei in the spinal cord lesion site of T10 wild-type (WT) mice at 2 weeks after SCI. Scale bar, 50 µm. Left images are high-resolution versions of the boxed regions in the right images. Scale bar, 10 µm. d Quantitative analysis of percentage of PDGFR-β cells/tdTomato + type A pericytes cells (*** P < 0.001, n = 4). e Representative images of immunofluorescent analysis of collagen III + (Col-III green) fibrotic scar, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in 13C4 group control mice and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. f Quantitative analysis of percentage of collagen III + area /tdTomato + type A pericytes field (*** P < 0.001, n = 4). g Representative images of immunofluorescent analysis of LepR + (green) MSCs, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in sham group mice, 13C4 group control mice, and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. h Quantitative analysis of the percentage of LepR + cells/tdTomato + cells (* P < 0.05, n = 4). i Representative images of immunofluorescent analysis of LepR + (green) MSCs, collagen III + (Col-III, green) fibrotic scar , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. j Quantitative analysis of the percentage of collagen III + area/ LepR + MSCs field (** P < 0.01, n = 4). k Representative images of immunofluorescent analysis of LepR + (green) MSCs, FSP1 + (red) type A pericytes , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. l Quantitative analysis of the percentage of LepR + MSCs/FSP1 + cells (** P < 0.01, n = 4). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Journal: Bone Research

Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

doi: 10.1038/s41413-026-00507-7

Figure Lengend Snippet: LepR + MSCs are the primary cells for fibrotic scar formation. a , b Diagram illustrating the genetic strategy to trace type A pericytes and the timeline of tamoxifen injection. c Representative images of immunofluorescent analysis of tdT + (red) type A pericytes, PDGFR-β + (green) pericytes, and DAPI (blue) staining of nuclei in the spinal cord lesion site of T10 wild-type (WT) mice at 2 weeks after SCI. Scale bar, 50 µm. Left images are high-resolution versions of the boxed regions in the right images. Scale bar, 10 µm. d Quantitative analysis of percentage of PDGFR-β cells/tdTomato + type A pericytes cells (*** P < 0.001, n = 4). e Representative images of immunofluorescent analysis of collagen III + (Col-III green) fibrotic scar, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in 13C4 group control mice and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. f Quantitative analysis of percentage of collagen III + area /tdTomato + type A pericytes field (*** P < 0.001, n = 4). g Representative images of immunofluorescent analysis of LepR + (green) MSCs, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in sham group mice, 13C4 group control mice, and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. h Quantitative analysis of the percentage of LepR + cells/tdTomato + cells (* P < 0.05, n = 4). i Representative images of immunofluorescent analysis of LepR + (green) MSCs, collagen III + (Col-III, green) fibrotic scar , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. j Quantitative analysis of the percentage of collagen III + area/ LepR + MSCs field (** P < 0.01, n = 4). k Representative images of immunofluorescent analysis of LepR + (green) MSCs, FSP1 + (red) type A pericytes , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. l Quantitative analysis of the percentage of LepR + MSCs/FSP1 + cells (** P < 0.01, n = 4). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Techniques: Injection, Staining, Control

Neonatal mice completely recover from SCI without scarring. a Schematic diagram illustrating the timeline of the experimental procedures. b Representative images of immunofluorescent analysis of pSmad2 (red), PDGFR-β (green), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2, 7, and 12 with or without SCI. Scale bar, 50 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. c , d Quantitative analysis of the number of pSmad2 + cells and PDGFR-β + cells (** P < 0.01, *** P < 0.001, **** P < 0.000 1, n = 6). e Representative images of immunofluorescent analysis of β-III-tubulin (green), collagen III (red), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2 and 12, and adult mice with or without SCI. Scale bar, 200 µm. f , g Quantitative analysis of the intensity mean value of collagen III + fibrotic scar and β-III-tubulin + nerves (* P < 0.05, **** P < 0.000 1, n = 6). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Journal: Bone Research

Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

doi: 10.1038/s41413-026-00507-7

Figure Lengend Snippet: Neonatal mice completely recover from SCI without scarring. a Schematic diagram illustrating the timeline of the experimental procedures. b Representative images of immunofluorescent analysis of pSmad2 (red), PDGFR-β (green), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2, 7, and 12 with or without SCI. Scale bar, 50 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. c , d Quantitative analysis of the number of pSmad2 + cells and PDGFR-β + cells (** P < 0.01, *** P < 0.001, **** P < 0.000 1, n = 6). e Representative images of immunofluorescent analysis of β-III-tubulin (green), collagen III (red), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2 and 12, and adult mice with or without SCI. Scale bar, 200 µm. f , g Quantitative analysis of the intensity mean value of collagen III + fibrotic scar and β-III-tubulin + nerves (* P < 0.05, **** P < 0.000 1, n = 6). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

Techniques:

Comparison of the relative expression of LOX and PDGFR in gastric cancer tissues through WB. (A) Relative expression of LOX: metastatic gastric cancer tissues (Lanes 1-3), nonmetastatic gastric cancer tissues (Lanes 4-5). (B) Relative expression of LOX: T3-T4 group (Lanes 1-2), T1-T2 group (Lanes 3-4). (C) Relative expression of PDGFR: metastatic gastric cancer tissues (Lanes 1-3), nonmetastatic gastric cancer tissues (Lanes 4-5). (D) Relative expression of PDGFR: T3-T4 group (Lanes 1-2), T1-T2 group (Lanes 3-4). (E) Relative expression of LOX in metastatic gastric cancer tissues was higher than in nonmetastatic gastric cancer tissues; the relative expression of LOX in the T3-T4 group was higher than in the T1-T2 group, P < 0.05. (F) Scatter plot showing that the relative expression of LOX was positively correlated with PDGFR in gastric cancer tissue ( r LOX-PGGFR = 0.634, P < 0.0001). (G) Bar charts show that the relative expression of PDGFR in metastatic gastric cancer tissues was higher than in nonmetastatic gastric cancer tissues; the relative expression of PDGFR in the T3-T4 group was higher than in the T1-T2 group, P < 0.05.

Journal: Journal of Cancer

Article Title: Lysyl Oxidase Promotes the Formation of Vasculogenic Mimicry in Gastric Cancer through PDGF-PDGFR Pathway

doi: 10.7150/jca.92192

Figure Lengend Snippet: Comparison of the relative expression of LOX and PDGFR in gastric cancer tissues through WB. (A) Relative expression of LOX: metastatic gastric cancer tissues (Lanes 1-3), nonmetastatic gastric cancer tissues (Lanes 4-5). (B) Relative expression of LOX: T3-T4 group (Lanes 1-2), T1-T2 group (Lanes 3-4). (C) Relative expression of PDGFR: metastatic gastric cancer tissues (Lanes 1-3), nonmetastatic gastric cancer tissues (Lanes 4-5). (D) Relative expression of PDGFR: T3-T4 group (Lanes 1-2), T1-T2 group (Lanes 3-4). (E) Relative expression of LOX in metastatic gastric cancer tissues was higher than in nonmetastatic gastric cancer tissues; the relative expression of LOX in the T3-T4 group was higher than in the T1-T2 group, P < 0.05. (F) Scatter plot showing that the relative expression of LOX was positively correlated with PDGFR in gastric cancer tissue ( r LOX-PGGFR = 0.634, P < 0.0001). (G) Bar charts show that the relative expression of PDGFR in metastatic gastric cancer tissues was higher than in nonmetastatic gastric cancer tissues; the relative expression of PDGFR in the T3-T4 group was higher than in the T1-T2 group, P < 0.05.

Article Snippet: They were incubated independently with 1:100 rabbit anti-LOX, 1:200 rabbit anti-PDGFR, 1:50 rabbit anti-PDGFRα, or 1:50 goat anti-PDGFRβ antibody (Santa Cruz Biotechnology), or 1:1000 rabbit anti-actin (Key GEN Bio TECH, Jiangsu Province, China) at room temperature for 3 h and then treated with HRP-labeled anti-rabbit or anti-goat IgG.

Techniques: Comparison, Expressing

LOX upregulated PDGFR expression in gastric cancer cells. (A) Results of Western blot show the relative expression of PDGFRα and PDGFRβ in gastric cancer cells treated with exogenous LOX. The LOX concentration was positively correlated with the relative expression of PDGFR. (B) Western blot shows the relative expression of PDGFRα and PDGFRβ in gastric cancer cells treated with BAPN. The concentration of BAPN was negatively correlated with the relative expression of PDGFR. Error bars represent SD, n = 5.

Journal: Journal of Cancer

Article Title: Lysyl Oxidase Promotes the Formation of Vasculogenic Mimicry in Gastric Cancer through PDGF-PDGFR Pathway

doi: 10.7150/jca.92192

Figure Lengend Snippet: LOX upregulated PDGFR expression in gastric cancer cells. (A) Results of Western blot show the relative expression of PDGFRα and PDGFRβ in gastric cancer cells treated with exogenous LOX. The LOX concentration was positively correlated with the relative expression of PDGFR. (B) Western blot shows the relative expression of PDGFRα and PDGFRβ in gastric cancer cells treated with BAPN. The concentration of BAPN was negatively correlated with the relative expression of PDGFR. Error bars represent SD, n = 5.

Techniques: Expressing, Western Blot, Concentration Assay

PDGFR inhibitors can suppress VM formation in gastric cancer cells. The VM were analyzed by two-dimensional culture of gastric cancer cells. (A) Different concentrations of PDGFR inhibitors significantly inhibited VM formation in gastric cancer cells (black arrow indicates VM formation; ×40); (B) Concentrations of PDGFR inhibitors were negatively correlated with the number of VM structures.

Journal: Journal of Cancer

Article Title: Lysyl Oxidase Promotes the Formation of Vasculogenic Mimicry in Gastric Cancer through PDGF-PDGFR Pathway

doi: 10.7150/jca.92192

Figure Lengend Snippet: PDGFR inhibitors can suppress VM formation in gastric cancer cells. The VM were analyzed by two-dimensional culture of gastric cancer cells. (A) Different concentrations of PDGFR inhibitors significantly inhibited VM formation in gastric cancer cells (black arrow indicates VM formation; ×40); (B) Concentrations of PDGFR inhibitors were negatively correlated with the number of VM structures.

Techniques:

Blocking PDGFR may partially inhibit the promotion of LOX on VM formation. The VM were analyzed by two-dimensional culture of gastric cancer cells. (A) VM formation of gastric cancer cells treated with LOX and 10 μM AG1295 or 5 μM AG1296 (black arrow indicates VM formation; ×40). The LOX concentration was positively correlated with the number of VM structures. (B) VM formation of gastric cancer cells treated with BAPN and 5 μM AG1296. (C) Comparison of VM formation with or without AG1295 and AG1296 at the same concentration of LOX.

Journal: Journal of Cancer

Article Title: Lysyl Oxidase Promotes the Formation of Vasculogenic Mimicry in Gastric Cancer through PDGF-PDGFR Pathway

doi: 10.7150/jca.92192

Figure Lengend Snippet: Blocking PDGFR may partially inhibit the promotion of LOX on VM formation. The VM were analyzed by two-dimensional culture of gastric cancer cells. (A) VM formation of gastric cancer cells treated with LOX and 10 μM AG1295 or 5 μM AG1296 (black arrow indicates VM formation; ×40). The LOX concentration was positively correlated with the number of VM structures. (B) VM formation of gastric cancer cells treated with BAPN and 5 μM AG1296. (C) Comparison of VM formation with or without AG1295 and AG1296 at the same concentration of LOX.

Techniques: Blocking Assay, Concentration Assay, Comparison

Relative protein expression of LOX and PDGFR in gastric cancer tissues detected using Western blotting

Journal: Journal of Cancer

Article Title: Lysyl Oxidase Promotes the Formation of Vasculogenic Mimicry in Gastric Cancer through PDGF-PDGFR Pathway

doi: 10.7150/jca.92192

Figure Lengend Snippet: Relative protein expression of LOX and PDGFR in gastric cancer tissues detected using Western blotting

Techniques: Expressing, Western Blot

Review

goat anti pdgfr β (R&D Systems)

Structured Review

Images

Related Articles

other:

Incubation:

Similar Products

Image Search Results